-Proteins
-DNA
-PCR
-Genetics
-Immunology
-Mycoplasma

Proteins
  • SDS-PAGE
  • Silver Stain
  • Western Blotting

    • DNA
  • Agarose gels
  • Plasmid DNA Isolation - alkaline lysis (B&D)
  • Mycoplasma DNA - Agarose method
  • Mycoplasma chromosomal DNA
  • DNA Isolation - general methods
  • DNA Glassmilk Isolation
  • M. hyopneumoniae PCR
  • Solutions
  • Elution of DNA from agarose
  • Kinase reactions
  • Phenol extraction of DNA
  • Precipitation of Nucleic Acids
  • Random libraries
  • Restriction digests
  • SmaI digestions
  • CTAB procedure

    • PCR
  • General PCR methods
  • M. hyopneumoniae PCR
  • Purification of PCR fragments

    • Genetics
  • Conjugation
  • Electroporation of E. coli
  • Overcoming restriction barriers in transformation
  • Media
  • Solutions

    • Immunology
  • ELISA
  • Hybridomas: expanding clones, etc.
  • Antisera

    • Mycoplasma
  • PPLO Broth
  • Other mycoplasma media
  • Friis Broth
  • Acid-adjusted Swine Serum
  • Yeast Extract Recipes
  • DNA in agarose
  • Chromosomal DNA in TE

    • Microarray